(VU Files Free Help) Assignment/GDB Updates
February 5, 2025 at 08:35 AM
*Name* π©βπΌ :
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*Subject* π :
Bio505T: Essential of Genetics
*Date* :ποΈ
04/02/2025
*Day* :
Tuesday
*Time* :
5:45 pm
*Paper duration* :
90 min
*No of Mcqs*:
40
*No of short questions*:
06
*No of Long questions*:
04
*Objective* : π«
1.In PCR annealing temperature is *40-68*
2.How many mechanisms involved in epigenetics? *6*
3.in Fish species that have four different alleles have number of genotypes *10*.
4.Northern blot is used to detect *RNA*
5.Southern blot is used to detect *DNA*
6.Mini PCR is related to *short primers*.
7.Number of genes in human mitochondrial genome *37*.
8.Which is the longest phase of Meiosis | *prophase*.
9.Mutation results in frame shift *duplication*. Handouts m addition hai lkin option m nai tha.wesy isy insertion b Kahty hai
10.Non-synonymus *25000*
11.Used for Identifying SNPs *Allele specific PCR*.
12.Large arm of chromosome is *Q arm*.
13. Ribose sugar +base *nucloside*.
14. Inherited variations *activity of genes*.
15. Which has largest number of chromosomes *horsetail*.
16. Chromatids separate and do not separate *mitosis and meiosis |*
17. Which is not molecular marker *SDS*.
18. If genes are linked then *recombination frequency is higher*.
19. Trisomy 21 :*47,XX,+21*
20. A ring chromosome is a chromosome whose...have fused together to form a ring.*arms*.
21. Which is not forward mutation related:* *mutation that changes a mutant phenotype back into wild type*.
22. Monozygotic identical twins ka symbol tha
23. Genetic problem 16 *either the baby belongs to her*.
24. Cell lysis *erythrocytes or leukocytes*
25. Dissolve RNA pellet by adding *RNAse-free* water to each sample.
*Subjective*:
*Short questions*;;:
Attempt any four questions.
1.Write about DNA loop in E.coli? (3 marks)
2.what are mutations ?write about its types? ( 3 marks)
3.Write the role of gene counselor in genetics?
4.Write down about organic procedure steps?
5.Difference between Hot start PCR and suicide PCR?
6.What is leukocytes lysis?
*Long question*;;
Attempt any 3 questions.
1.What in VNTR ? Write at least it's 5 applications? ( 5 marks)
2.Difference between AFL polymorphism and RAP DNA?
3.Difference between Mitosis and Meiosis with respect to DNA replication, divisions, synapis of homologous chromosomes, no of daughter cells produced, role in animals?
4.What is western blotting technique? Write it's procedure.
*Note*: