RPR preparation@TricellBiologics [not advertisement related, only for information] Double spin with 25ml blood (1st 2400 rpm for 10min; 2nd 3300 rpm for 5min). Do you use single or double spin in your case ? In testuben or PRP tubes or specialized container like in this video? What is your centrifugation specitications? How much blood you use and how much is the PRP yield, and most importantly whats the count you use?